Panel Design for Immunophenotyping

Article Collection: Demystifying Panel Design for Immunophenotyping

Few things in the world of flow cytometry can appear more daunting to a novice user than the design of immunophenotyping panels for deep characterization of cell types. Considerations such as, overlap of emission spectra and prevalence of identifying cell markers are key to the design of immunophenotyping panels.

With advances in flow cytometry such as spectral flow cytometry and the wide range of fluorophores available to researchers, panels can now be redesigned or optimized to also identify subpopulations of cells or various states of activation to increase the depth of information acquired.

By introducing readers to the process of panel design and optimization underlying effective immunophenotyping by flow cytometry, we hope to empower users to develop panels that address their specific research or diagnostic goals.

This article collection highlights effective immunophenotyping panel design from conception to optimization to aid researchers and demystify the process.

What you will learn:

Overview of the panel design, optimization, and validation process
How to increase the depth of information that is acquired through effective panel design
Examples of the panel design process in action

Articles contained in the collection:

Ferrer‐Font et al. (2020). Panel design and optimization for high‐dimensional immunophenotyping assays using spectral flow cytometry. Current Protocols in Cytometry
Tembhare et al. (2017). Method for DNA ploidy analysis along with immunophenotyping for rare populations in a sample using FxCycle violet. Current Protocols in Cytometry
Payne et al. (2020). OMIP-063: 28-Color Flow Cytometry Panel for Broad Human Immunophenotyping. Cytometry
Hertoghs et al. (2020). OMIP-064: A 27-Color Flow Cytometry Panel to Detect and Characterize Human NK Cells and Other Innate Lymphoid Cell Subsets, MAIT Cells, and γδ T Cells. Cytometry
Ohne, Y. (2020). OMIP-066: Identification of Novel Subpopulations of Human Group 2 Innate Lymphoid Cells in Peripheral Blood. Cytometry
Nogimori et al. (2021). OMIP 078: A 31-parameter panel for comprehensive immunophenotyping of multiple immune cells in human peripheral blood mononuclear cells. Cytometry

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